As of January 1, 2024, the following United States Food and Drug Administration (FDA)-approved tests are available for use in the HIV Diagnostic Testing Algorithm for laboratories.

Laboratories should use this same testing algorithm, beginning with an antigen/antibody immunoassay on all serum or plasma specimens submitted for testing after a preliminary positive result from any …

HIVSS / HIV-1 and HIV-2 Antigen and Antibody Routine Screen, Serum If indicated, all subsequent tests listed are automatically performed and charged separately.

Clinicians should offer repeat HIV testing every 3 months, or sooner if acute HIV is suspected, for as long as an individual remains at high risk of HIV exposure.

Apr 1, 2025 · In 2014, the CDC and the Association of Public Health Laboratories (APHL) released an HIV diagnostic algorithm to allow for more accurate diagnosis of acute HIV-1, improved ability to …

IV LABORATORY DIAGNOSTIC TESTING ALGORITHM The HIV Laboratory Diagnostic Testing Algorithm1 should be used for testing serum or plasma to diagnose persons with HIV and for the …

Per the standard HIV laboratory testing algorithm, if a reactive result is obtained with an HIV-1/2 Ag/Ab immunoassay test (step 1), clinicians should perform supplemental testing (step 2) with an FDA …

APHL and CDC continue to recommend that laboratories use an FDA-approved instrumented HIV-1/HIV-2 antigen/antibody immunoassays as the initial assay the initial assay in the laboratory HIV …

Sep 25, 2025 · Provides updated recommendations for HIV testing by laboratories in the United States and describes approaches for reporting test results to persons ordering HIV tests and to public health …

The following testing recommendations are from current Association of Public Health Laboratories (APHL) and Centers for Disease Control and Prevention (CDC) guidelines.